KMID : 0380620210530020218
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Korean Journal of Food Science and Technology 2021 Volume.53 No. 2 p.218 ~ p.222
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Effect of UV-C irradiation for bacterial disinfection of drinking water
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Kim Hyun-Joong
Kim Yun-Jae Seo Se-Yeong Lee Chang-Joo
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Abstract
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This study aimed to investigate bacterial disinfection in drinking water using a water purifier. Water artificially inoculated with Escherichia coli and Listeria monocytogenes at various concentrations was irradiated using ultraviolet (UV)-C at a rate of 3.4 L/min in a water purifier, and the disinfection effects of UV-C were evaluated. Both E. coli and L. monocytogenes were disinfected up to 107 colony-forming units (CFU)/2.8 L by the UV-C irradiation. Additionally, morphological study using fluorescence microscopy in conjunction with live/dead staining revealed that both the bacteria species were disinfected by the UV-C irradiation. Therefore, UV-C in water purifiers can effectively kill high concentrations of bacteria in distilled water. UV irradiation (UV-C: 254 nm wavelength, irradiation dose: 40 mJ/cm2) at a flow rate of 3.4 L/min on drinking water has the potential to sterilize bacteria-contaminated drinking water, at least for 3.2¡¿107 CFU/2.8 L of E. coli and 8.4¡¿107 CFU/2.8 L of L. monocytogenes.
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KEYWORD
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UV-C, sterilization, disinfection, Gram positive, Gram negative
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